The original paper is in English. Non-English content has been machine-translated and may contain typographical errors or mistranslations. ex. Some numerals are expressed as "XNUMX".
Copyrights notice
The original paper is in English. Non-English content has been machine-translated and may contain typographical errors or mistranslations. Copyrights notice
Foi desenvolvido um conjunto de microrreatores para uso bioquímico ou biomédico. O conceito deste desenvolvimento é obter o máximo de dados biológicos ao mesmo tempo. Noventa e seis micropoços de reação, com volume de cada poço de 1.5 µl, foram integrados na matriz. O conjunto de microrreatores foi fabricado em pastilha de silício com 1 mm de espessura e doze pares de um sensor de temperatura e um aquecedor foram formados na parte traseira. Uma pequena janela transparente para medição óptica foi formada no centro da parede inferior de cada poço. Vários gradientes de temperatura foram aplicados ao arranjo por meio de poucos aquecedores e comparados com resultados de simulação para otimizar os parâmetros. Finalmente, o desempenho do array foi avaliado pela reação básica do DNA. As vantagens do sistema array são a rápida resposta térmica devido à pequena capacidade térmica e a facilidade de realizar diversas condições de reação em paralelo.
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Etsuo SHINOHARA, Seiji KONDO, Kouki AKAHORI, Kohichi TASHIRO, Shuichi SHOJI, "Development of Biological Micro Reactor Array System" in IEICE TRANSACTIONS on Electronics,
vol. E84-C, no. 12, pp. 1807-1813, December 2001, doi: .
Abstract: A micro reactor array for biochemical or biomedical use was developed. Conceptof this development is to get as much as biological data at the same time. Ninety-six micro reaction wells, volume of each well was 1.5 µl, were integrated in the array. The micro reactor array was fabricated on 1 mm thick silicon wafer and twelve pairs of a temperature sensor and a heater were formed on the backside. A tiny transparent window for optical measurement was formed at the center of bottom wall on each well. Several temperature gradients were applied to the array by means of few heaters and compared with simulation results to optimize the parameters. Finally, performance of the array was evaluated by basic DNA reaction. Advantages of the array system are the fast thermal response due to the small heat capacity and easy to make several reaction conditions in parallel.
URL: https://global.ieice.org/en_transactions/electronics/10.1587/e84-c_12_1807/_p
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@ARTICLE{e84-c_12_1807,
author={Etsuo SHINOHARA, Seiji KONDO, Kouki AKAHORI, Kohichi TASHIRO, Shuichi SHOJI, },
journal={IEICE TRANSACTIONS on Electronics},
title={Development of Biological Micro Reactor Array System},
year={2001},
volume={E84-C},
number={12},
pages={1807-1813},
abstract={A micro reactor array for biochemical or biomedical use was developed. Conceptof this development is to get as much as biological data at the same time. Ninety-six micro reaction wells, volume of each well was 1.5 µl, were integrated in the array. The micro reactor array was fabricated on 1 mm thick silicon wafer and twelve pairs of a temperature sensor and a heater were formed on the backside. A tiny transparent window for optical measurement was formed at the center of bottom wall on each well. Several temperature gradients were applied to the array by means of few heaters and compared with simulation results to optimize the parameters. Finally, performance of the array was evaluated by basic DNA reaction. Advantages of the array system are the fast thermal response due to the small heat capacity and easy to make several reaction conditions in parallel.},
keywords={},
doi={},
ISSN={},
month={December},}
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TY - JOUR
TI - Development of Biological Micro Reactor Array System
T2 - IEICE TRANSACTIONS on Electronics
SP - 1807
EP - 1813
AU - Etsuo SHINOHARA
AU - Seiji KONDO
AU - Kouki AKAHORI
AU - Kohichi TASHIRO
AU - Shuichi SHOJI
PY - 2001
DO -
JO - IEICE TRANSACTIONS on Electronics
SN -
VL - E84-C
IS - 12
JA - IEICE TRANSACTIONS on Electronics
Y1 - December 2001
AB - A micro reactor array for biochemical or biomedical use was developed. Conceptof this development is to get as much as biological data at the same time. Ninety-six micro reaction wells, volume of each well was 1.5 µl, were integrated in the array. The micro reactor array was fabricated on 1 mm thick silicon wafer and twelve pairs of a temperature sensor and a heater were formed on the backside. A tiny transparent window for optical measurement was formed at the center of bottom wall on each well. Several temperature gradients were applied to the array by means of few heaters and compared with simulation results to optimize the parameters. Finally, performance of the array was evaluated by basic DNA reaction. Advantages of the array system are the fast thermal response due to the small heat capacity and easy to make several reaction conditions in parallel.
ER -